15038 studies
Thymosin alpha 1, an immune restorative polypeptide hormone, was synthesized in Escherichia coli by using recombinant DNA cloning techniques. Based on the known amino acid sequence, a gene coding for the thymosin alpha 1 polypeptide chain was designe…
Turnip isoperoxidase TP 7 had its hemin group removed and was cleaved by trypsin. The digest was fractionated by gel filtration and high-voltage paper electrophoresis and yielded 24 peptides, which counted for all 296 amino acid residues of the enzym…
The complete amino acid sequence of turnip peroxidase TP 7, the principal isoperoxidase during winter in turnip, Brassica napus L., variety Blanc dur d'hiver, has been determined by sequence analysis of cyanogen bromide fragments and of tryptic pepti…
Primary cultures of rat thyroid cells were made in medium supplemented with 0.1--0.5% calf serum and containing six hormones or growth factors: insulin, thyrotropin, transferrin, hydrocortisone, somatostatin, and glycyl-L-histidyl-L-lysine acetate. T…
The growth promoting activity of a synthesis tripeptide, glycyl-L-histidyl-L-lysine (GHL) as a possible serum substitute was determined using human KB cells. GHL was found to stimulate the growth of human KB, and HeLa cells, but not WI-38 cells when…
The objective of the present study was to determine whether or not thymosin and conditioned medium from human thymus epithelial cultures (HTECM) contain similar fractions, capable of inducing T-cell differentiation. Therefore we tested the ability of…
Thymosin fraction 5 contains several distinct hormonal-like factors which are effective in partially or fully inducing and maintaining immune function. Several of the peptide components of fraction 5 have been purified, sequenced and studied in assay…
Benefit/risk factors in immunization against poliomyelitis are examined from the viewpoint of the relative risk and efficacy of killed and live poliovirus vaccines in the currently changing contexts of poliomyelitis prevalence in developed and develo…
The authors report the results of experimental and clinical estimation of the effect of polypeptide extract of the epiphysis (epithalamin) on cervical cancer. Injection of epithalamin is shown to improve the cell immunity indices and renders an antit…
In the present investigation the occurrence of humoral immunity to pancreatic duct cells (PDC) was studied in 12 patients with Sjögren's syndrome (SS), 31 patients with rheumatoid arthritis (RA), and 64 controls. Four sera of patients with SS and eig…
Proton magnetic resonance studies of the acid-base chemistry of the glycyl ammonium, histidyl imidazolium, and lysyl ammonium groups of glycyl-L-histidyl-L-lysine and of the glycyl ammonium and histidyl imidazolium groups of glycyl-L-histidine and gl…
Rat and calf adrenal cortex homogenates were found to contain three different malic enzymes. Two were strictly NADP+-dependent and were localized, one each, in the cytosol and the mitochondrial fractions, respectively. These two enzymes appear to be…
Daily treatment of hemicastrated young rats with 0.57 mug of stilbestrol inhibited compensatory ovarian hypertrophy (COH) by 48% in the 3-month animals and by 3% only in the 18-month animals. Administration of L-DOPA, dilantin, epithalamin or phenofo…