Polydopamine-coated 3D electrospun sponge conjugated with thrombin receptor-activating peptide and antibacterial peptide for hemostasis and accelerated healing of infected wounds.

Due to the complex and variable microenvironment of wounds, bleeding and persistent infections have always posed significant challenges in wound treatment. Designing and developing therapeutic solutions with rapid hemostatic capabilities and antibacterial activity is crucial for effective tissue repair. In this study, we developed a novel three-dimensional electrospun sponge dressing that is surface-modified with polydopamine (PDA) and conjugated with thrombin receptor-activating peptide (TRAP) and the antibacterial peptide LL-37. This 3D sponge significantly enhances hydrophilicity and mechanical properties while maintaining high porosity and water absorption. Additionally, the sponge exhibits excellent cell compatibility, antibacterial properties, and in vitro coagulation performance. In liver hemostasis models and femoral artery hemostasis models, this sponge effectively shortened hemostasis time and reduced blood loss. Furthermore, in infected wound rat models, this material not only accelerated wound closure but also demonstrated strong antibacterial properties and tissue regeneration capabilities. This study presents a novel sponge dressing with translational potential for the management of clinical bleeding and infected wounds.