Ability of Linezolid to CombatandIsolated from Polymicrobial Wound Infections.

: The optimal therapy for polymicrobial wound infections is poorly defined. We sought to characterize the ability of linezolid to combat mixed cultures ofand.: The antistaphylococcal activity of linezolid was assessed in 24-h time-killing experiments that usedandisolated from polymicrobial wound infections. Clindamycin was also evaluated as a comparator. A Hill-type mathematical model was used to assess the maximum killing of(E). The ability of linezolid to potentiate the activity of host defense peptides againstwas evaluated using LL-37.: In the presence of, the Eof linezolid decreased in 5/9 co-culture experiments and increased in 4/9 co-culture experiments in comparison to linezolid againstalone. The potency of linezolid was not significantly impacted by the presence of. In comparison, the maximalkilling achieved by clindamycin decreased in eight out of nine experiments, and somewhat paradoxically, the potency increased in nine out of nine experiments. In the host defense peptide assay, the supratherapeutic linezolid concentration of 64 mg/L did not significantly enhance the killing of the LL-37 peptides (≥ 0.121), but the concentration of linezolid was significantly associated with the killing of one of threeisolates (= 0.005).:had a minimal impact on the antistaphylococcal activity of linezolid in comparison to clindamycin. Linezolid did not exert a consistent ability to enhance the antipseudomonal activity of host defense peptides. These data may help inform antimicrobial selection during polymicrobial wound infections.