Efficacy of Bacteriophages in-Induced Inflammation in Mice.

BACKGROUND: Bacteriophages have been introduced as living drugs for infectious diseases; thus, they may provide an alternative to conventional acne therapeutics in patients with non-responsive acne. OBJECTIVE: We investigated the effect of bacteriophages using an acne mouse model with-induced inflammatory nodules by clinical examination, pathology, and immunohistochemical analysis. METHODS: A human-isolatedsuspension (10colony forming units/µl) was injected into the backs of HR-1 mice. Group A was used as a control, Group B was injected on the back with4 weeks following the initialsuspension injection, and group C was injected on the back withand bacteriophages 4 weeks following the initialsuspension injection. Clinical and histopathological evaluations were performed. RESULTS: Inflammatory nodule size decreased with time in all groups. Group C showed the greatest decrease in size, followed by group B and group A. The histopathological findings showed a decrease in epidermal thickness and the number and size of microcomedone-like cysts in groups B and C compared to group A. Immunohistochemistry revealed similar expression of integrin α6, the epidermal proliferation marker, infiltration of CD4/CD8 T cells and neutrophils, and expression of myeloperoxidase, interleukin-1β, toll-like receptor-2, LL-37, and matrix metalloproteinase-2/3/9 in all three groups. CONCLUSION: Using an acne mouse model with-induced inflammatory nodules, we demonstrate that bacteriophages may constitute an alternative to conventional acne therapies. However, additional studies are needed for human applications.