Adsorption effects of the doping relevant peptides Insulin Lispro, Synachten, TB-500 and GHRP 5.

Analytical biochemistry2017PMID: 28887173

View on PubMed DOI: 10.1016/j.ab.2017.09.003

Plain Language Summary

Analyzes adsorption of four doping-relevant peptides—GHRP-5, TB-500 (the Ac-LKKTETQ fragment of thymosin β4), insulin lispro, and synacthen—to various analytical surfaces. TB-500 showed significant surface adsorption that varied substantially between container materials; expensive low-bind plasticware did not universally prevent losses. Identifies optimal container choices for TB-500 recovery—critical practical data for anti-doping laboratories performing accurate TB-500 quantification at trace concentrations required for doping control.

Abstract

The tendency of peptides to adsorb to surfaces can raise a concern in variety of analytical fields where the qualitative/quantitative measurement of low concentration analytes (ng/mL-pg/mL) is required. To demonstrate the importance of using the optimal glassware/plasticware, four doping relevant model peptides (GHRP 5, TB-500, Insulin Lispro, Synachten) were chosen and their recovery from various surfaces were evaluated. Our experiments showed that choosing expensive consumables with low-bind characteristics is not beneficial in all cases. A careful selection of the consumables based on the evaluation of the physico/chemical features of the peptide is recommended.

Authors

Judák, Péter; Van Eenoo, Peter; Deventer, Koen

Keywords

AdsorptionDoping controlLow-bindPeptide