Cerebral toxoplasmosis is characterized by activation of brain resident cells and recruitment of specific immune cell subsets from the periphery to the central nervous system (CNS). Our studies revealed that the rapidly invaded Ly6Gneutrophil granulocytes are an early non-lymphoid source of interferon-gamma (IFN-γ), the cytokine known to be the major mediator of host resistance to(). Upon selective depletion of Ly6Gneutrophils, we detected reduced IFN-γ production and increased parasite burden in the CNS. Ablation of Ly6Gcells resulted in diminished recruitment of Ly6Cmonocytes into the CNS, indicating a pronounced interplay. Additionally, we identified infiltrated Ly6Gneutrophils to be a heterogeneous population. The Ly6GCD62-LCXCR4subset released cathelicidin-related antimicrobial peptide (CRAMP), which can promote monocyte dynamics. On the other hand, the Ly6GCD62-LCXCR4subset produced IFN-γ to establish early inflammatory response. Collectively, our findings revealed that the recruited Ly6GCXCR4neutrophil granulocytes display a heterogeneity in the CNS with a repertoire of effector functions crucial in parasite control and immune regulation upon experimental cerebral toxoplasmosis.