Plasma levels of F-actin and F:G-actin ratio as potential new biomarkers in patients with septic shock.

Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals2016PMID: 26754286

View on PubMed DOI: 10.3109/1354750X.2015.1126646

Plain Language Summary

Measures plasma F-actin, G-actin, and thymosin β4 in septic shock, noninfectious SIRS, and healthy controls. F-actin was significantly elevated in septic shock; G-actin was highest in noninfectious SIRS. Notably, TB4 was not detectable above assay limits in the septic shock group—suggesting TB4 is consumed or complexed in severe systemic inflammation. Identifies F-actin/G-actin ratio as a potential septic shock diagnostic biomarker and documents TB4 depletion in sepsis—providing the foundational biomarker data later expanded in PMID 32421363.

Abstract

OBJECTIVE: To compare plasma levels of F-actin, G-actin and thymosin beta 4 (TB4) in humans with septic shock, noninfectious systemic inflammatory response syndrome (SIRS) and healthy controls. RESULTS: F-actin was significantly elevated in septic shock as compared with noninfectious SIRS and healthy controls. G-actin levels were greatest in the noninfectious SIRS group but significantly elevated in septic shock as compared with healthy controls. TB4 was not detectable in the septic shock or noninfectious SIRS group above the assay's lowest detection range (78 ng/ml). CONCLUSIONS: F-actin is significantly elevated in patients with septic shock as compared with noninfectious SIRS. F-actin and the F:G-actin ratio are potential biomarkers for the diagnosis of septic shock.

Authors

Belsky, Justin B; Morris, Daniel C; Bouchebl, Ralph; Filbin, Michael R; Bobbitt, Kevin R; Jaehne, Anja K; Rivers, Emanuel P

Keywords

ActinF-actinG-actinactin binding proteincoronary artery bypass graftseptic shockthymosin beta 4