The presence and localization of cathelicidin anti-microbial peptides in the lizard, Anolis carolinensis, were investigated by immunocytochemistry. The study showed that immunoreactivity for cathelicidins 1 and 2 was only present in large granules of heterophilic-basophilic granulocytes, rarely found in the dermis and sub-dermal muscle in normal and more frequently in wound and regenerating skin tissues or in the blood. Some immunopositive granulocytes were also observed among the keratinocytes of the wound epithelium covering the tail stump and occasionally in the regenerating epidermis of the tail. Immunolabeling for cathelicidins was also seen in low electrondense amorphous material present on the surface of the wound epidermis and on the plasma membrane of bacteria present on the surface of corneocytes of the epidermis. Immunolabeling for cathelicidins was absent in the other cell types and in control sections. The study suggests that cathelicidins are normally stored in granulocytes in the blood or in connective tissues, while keratinocytes can be stimulated to produce and possibly release these molecules only after injury or microbial invasion.