Effects of peptide T derivatives on the proliferation of cultured human keratinocytes.

Arzneimittel-Forschung1995PMID: 7575755

Plain Language Summary

Derivatives of peptide T, a small protein fragment, were found to stimulate the growth of normal human skin cells (keratinocytes) in laboratory cultures. Experiments with a VIP-blocking agent showed that peptide T compounds and VIP likely promote skin cell growth through different cellular receptors, despite producing similar effects.

Abstract

[D-Ala1]peptideT-amide, the linear hexapeptide H-Thr-Hse-Asn-Tyr-Thr-Asp-OH (LPT) and its cyclic analog, cyclo(-Thr-Hse-Asn-Tyr-Thr-Asp-) (CPT), were tested for their effects on the proliferation of cultured normal human keratinocytes (KTs) in comparison with vasoactive intestinal peptide (VIP). [D-Ala1]PT-NH2, LPT and VIP (all 0.1 mumol/l) increased the cell number in KT cultures, whereas CPT was ineffective. The VIP antagonist [N-Ac-Tyr1,D-Phe2]GRF (1-29)-NH2 significantly inhibited the VIP effects on KTs. On the other hand this antagonist did not affect the peptide T (PT) compounds-induced stimulation of KTs, providing indirect evidence that the mitogenic effects of VIP and PT peptides are probably mediated via different receptors.

Authors

Marastoni, M; Scaranari, V; Fantini, F; Sevignani, C; Tomatis, R