[Recombinant thymosin alpha1].

Bioorganicheskaia khimiia2004PMID: 15562968

Plain Language Summary

Scientists developed a method to produce recombinant thymosin-alpha-1 in E. coli bacteria by creating an artificial gene fused with a yeast intein sequence. The hybrid protein was successfully expressed at high levels and could be cleaved to release active thymosin-alpha-1, providing a practical approach for manufacturing this immune-modulating peptide.

Abstract

An artificial gene encoding thymosin alpha1 was obtained by the chemoenzymatic synthesis and cloned into Escherichia coli. An expressing recombinant plasmid containing the hybrid protein gene, which encodes amino acid sequences of thymosin alpha1 and the Saccharomyces cerevisiae intein Sce VMA, was constructed. The expression of the hybrid protein from the resulting hybrid gene in E. coli, the properties of the resulting hybrid protein, and the conditions for its nonenzymatic cleavage to thymosin alpha1 were studied. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 5; see also http: // www.maik.ru.

Authors

Esipov, R S; Gurevich, A I; Stepanenko, V N; Chupova, L A; Chuvikovskiĭ, D V; Miroshnikov, A I