Studies of the effects of Vilon and Epithalon on gene expression in mouse heart using DNA-microarray technology.

Bulletin of experimental biology and medicine2002PMID: 12360356

Animal StudyEpitalon

Plain Language Summary

Using DNA microarray technology to study over 15,000 genes, researchers found that Epitalon altered expression of 98 genes in mouse heart tissue, while Vilon affected 36 genes. Combined treatment changed 144 genes, with both peptides predominantly activating rather than suppressing gene expression, demonstrating their broad but specific effects on cardiac gene regulation.

Abstract

Expression of 15,247 clones from a cDNA library in the heart of mice receiving Vilon and Epithalon was studied by DNA-microarray technology. We revealed 300 clones (1.94% of the total count), whose expression changed more than by 2 times. Vilon changed expression of 36 clones, while Epithalon modulated expression of 98 clones. Combined treatment with Vilon and Epithalon changed expression of 144 clones. Vilon alone or in combination with Epithalon activated expression of 157 clones (maximally by 6.13 times) and inhibited expression of 23 clones (maximally by 2.79 times). Epithalon alone or in combination with Vilon activated expression of 194 clones (maximally by 6.61 times) and inhibited expression of 48 clones (maximally by 2.71 times). Our results demonstrate the specific effects of Epithalon and Vilon on gene expression.

Authors

Anisimov, S V; Bokheler, K R; Khavinson, V Kh; Anisimov, V N